Comparison of Aspergillus fumigatus and Aspergillus umbrosus Antigens in Serological Tests of Farmer's Lung
- 1 July 1982
- Vol. 37 (5), 297-301
- https://doi.org/10.1111/j.1398-9995.1982.tb01915.x
Abstract
A comparison was made between A. fumigatus and A. umbrosus antigens regarding their usefulness in serological tests of farmer''s lung. Mycelial and culture fluid antigens of both microbes were used to test sera of 30 Finnish farmer''s lung patients for antibodies in the ELISA [enzyme-linked immunosorbent assay] and in the gel precipitation (GP) test. Antibodies to A. umbrosus were more common than to A. fumigatus; this was especially evident in ELISA. ELISA gave more positive results with all 4 antigens than GP. Mycelial antigens showed more positive reactions in ELISA than the corresponding culture fluid antigens. The situation was reversed in GP. Selection of antigens in the diagnostic panel of farmer''s lung requires careful consideration. Other Aspergilli (in the Finnish population A. umbrosus) might be preferable to A. fumigatus in the antigen panel.Keywords
This publication has 5 references indexed in Scilit:
- The Use of Enzyme‐Linked Immunosorbent Assay (ELISA) in the Diagnosis of Farmer's LungAllergy, 1980
- Antibodies to Aspergillus fumigatus in farmers' lung patients measured by enzyme‐linked immunosorbent assay (ELISA)Clinical and Experimental Allergy, 1980
- Microbiological and serological studies of farmers' lung in FinlandClinical and Experimental Allergy, 1979
- Precipitin (F.L.H.) Test in Farmer's LungThorax, 1965
- A Microplate Technique Employing a Gel Chamber Compared with Other Micro- and Macroplate Techniques for Immune DiffusionInternational Archives of Allergy and Immunology, 1962