Studies on the tissue specificity of the high-mobility-group non-histone chromosomal proteins from calf

Abstract
The high-mobility-group (HMG) non-histone chromosomal proteins from calf thymus, liver, spleen and kidney were extracted, and fractionated by CM-Sephadex chromatography and trichloroacetic acid precipitation. The isolated proteins HMG 1, HMG 2 and HMG 17 from the tissues were compared by polyacrylamide-gel electrophoresis, isoelectric focusing and amino acid analysis. The 3 proteins are very similar in the tissues studied, implying a lack of tissue specificity.