Plasmid ColE1 DNA replication in Escherichia coli strains temperature-sensitive for DNA replication
- 1 December 1975
- journal article
- research article
- Published by Springer Nature in Molecular Genetics and Genomics
- Vol. 136 (4), 273-289
- https://doi.org/10.1007/bf00341713
Abstract
Summary Mutants of the dnaA, dnaC, dnaD, polC, dnaF and dnaG gene loci were tested for their capacity for colicinogenic plasmid E1 (ColE1) replication at a non-permissive temperature. It was found that ColE1 replication was independent of the dnaA gene function and dependent on dnaC, D, F and G. ColE1 replication in the polC mutant E486 continued for several hours but at a greatly reduced rate. No effect was found of the dnaG mutation on thymine-deprivation-induced “priming” of ColE1 replication at the non-permissive temperature. The mutants also were tested for aberrant replication intermediates of plasmid DNA as well as a temperature sensitive supercoiled DNA-protein relaxation complex. RNA-containing supercoils were found to accumulated in a polC mutant also blocked for protein synthesis.Keywords
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