Purification and Isolation of Rheumatoid Factor
- 1 December 1958
- journal article
- research article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 99 (3), 554-555
- https://doi.org/10.3181/00379727-99-24417
Abstract
A new method for the purification of the rheumatoid factor is presented. A euglobulin fraction of serum was chromato-graphed on DEAE cellulose. The resultinting fraction was eluted from CMC cellulose with 0.1 [image] phosphate pH 4.4 to yield a preparation containing more than 90% of a component with a sedimentation coefficient of 18.8. Relative specific activities at successive stages of purification were determined. Concentration dependence data for the sedimentation coefficient were obtained.Keywords
This publication has 2 references indexed in Scilit:
- INTERACTION OF THE RHEUMATOID FACTOR WITH ANTIGEN-ANTIBODY COMPLEXES AND AGGREGATED GAMMA GLOBULINThe Journal of Experimental Medicine, 1958
- Differential Agglutination of Normal and Sensitized Sheep Erythrocytes by Sera of Patients with Rheumatoid ArthritisExperimental Biology and Medicine, 1948