Alloantigens Derived from Stimulator Cells and Bound onto M LC-activated Rat T Lymphoblasts
- 1 June 1979
- journal article
- research article
- Published by Wiley in Scandinavian Journal of Immunology
- Vol. 9 (6), 553-562
- https://doi.org/10.1111/j.1365-3083.1979.tb03284.x
Abstract
Rat T lymphocytes were activated in MLC (mixed leucocyte culture) against allogeneic stimulator cells. A few percent of such T blasts could be shown to carry IgM on their surface when a mixture of T and B lymphocytes was used as responder cells. The immunoglobulin was absent when purified T lymphocytes served as responders, A large proportion of MLC T lymphoblasts could be shown to carry stimulator alloantigens on their surface. These antigens were demonstrated (a) by indirect immunofluorescence using anti-stimulator alloantibody and FITC (fluorescein isothiocyanate Isomer I)-labelled F(ab)2 fragments of rabbit anti-rat Ig; (b) by autoradiography after internal labelling of stimulator cells. which showed the transfer of label from stimulator to responder cells. For biochemical analysis T blasts were restimulated with internally labelled allogeneic or syngeneic stimulator cells, and bound radiolabelled stimulator alloantigens were analysed on SDS-PAGE (sodium lauryl sulphate polyacrylamide gel electrophoresis). The results suggest that intact SD antigens with a molecular weight of 45,000 daltons and LD determinants with a molecular weight of 34,000 daltons derived from stimulator cells are found on MLC T blasts. No evidence of binding of the 27,000-dalton la chain to T blasts was found. Possible arrangements of these molecules on the responder blasts is discussed.This publication has 39 references indexed in Scilit:
- Partial Characterization of Cell Surface Idiotypes on Alloantigen‐Activated T LymphoblastsScandinavian Journal of Immunology, 1978
- Isolation and Partial Characterization of Rat Lymphoid Cell Surface Histocompatibility Antigens and ImmunoglobulinsScandinavian Journal of Immunology, 1978
- Idiotypic analysis of lymphocytes in vitro. II. Genetic control of T-helper cell responsiveness to anti-idiotypic antibody.The Journal of Experimental Medicine, 1976
- Localization of aggregated cell surface antigens of target cells bound to cytotoxic T lymphocytes.The Journal of Experimental Medicine, 1975
- Shared idiotypic determinants on B and T lymphocytes reactive against the same antigenic determinants. II. Determination of frequency and characteristics of idiotypic T and B lymphocytes in normal rats using direct visualization.The Journal of Experimental Medicine, 1975
- Immunoglobulin-Positive Cells: Their Appearance in the Mixed Lymphocyte ReactionScience, 1975
- Early cellular events in a systemic graft-vs.-host reaction. II. Autoradiographic estimates of the frequency of donor lymphocytes which respond to each Ag-B-determined antigenic complex.The Journal of Experimental Medicine, 1975
- Immunoglobulin on activated T cells detected by indirect immunofluorescenceCellular Immunology, 1974
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- A solubilizable acrylamide gel for electrophoresisFEBS Letters, 1970