Isolation of monoclonal antibodies specific for products of avian oncogene myb.
Open Access
- 1 December 1984
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 4 (12), 2843-2850
- https://doi.org/10.1128/mcb.4.12.2843
Abstract
We isolated a series of monoclonal antibodies which were raised against a bacterially expressed protein, bp37v-myb, and coded for by part of the avian v-myb gene. These monoclonal antibodies recognized a range of antigenic specificities on bp37v-myb, and this was reflected in their differing specificities for the gene products of the v-myb, c-myb, and E26 viral oncogenes. One monoclonal antibody recognized, in addition to the v-myb and c-myb gene products, a conserved nuclear protein found in all tested cells. We describe the characterization of these monoclonal antibodies.This publication has 20 references indexed in Scilit:
- A putative second cell-derived oncogene of the avian leukaemia retrovirus E26Nature, 1983
- Tripartite structure of the avian erythroblastosis virus E26 transforming geneNature, 1983
- Antibodies against synthetic peptides as a tool for functional analysis of the transforming protein pp60srcCell, 1983
- The product of the retroviral transforming gene v-myb is a truncated version of the protein encoded by the cellular oncogene c-mybCell, 1983
- A monoclonal anti‐HLA antibody recognizes a mouse tumor‐associated antigenEuropean Journal of Immunology, 1982
- Nucleotide sequence of the retroviral leukemia gene v-myb and its cellular progenitor c-myb: The architecture of a transduced oncogeneCell, 1982
- Production of monoclonal antibodies: Strategy and tacticsJournal of Immunological Methods, 1980
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-SepharoseImmunochemistry, 1978
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970