Migration of Mouse Lymphocytes in Vitro from Capillary Tube Cultures

Abstract

Summary Cell suspensions from mouse lymphoid organs, including the spleen, deep and superficial lymph nodes, bone marrow, and thymus, and from induced peritoneal exudates, were compared as regards their ability to migrate from capillary tubes under conditions of tissue cultures. Cells from all lymphoid tissues migrated rapidly in vitro. Purified lymphocyte suspensions prepared by glass bead column or glass plate adsorption procedures containing few, if any, macrophages migrated just as well as unfractionated cell suspensions. The utilization of lymphocytes for migration procedures in vitro for various biologic, immunologic, and physiologic studies are discussed. We are grateful to Dr. Maurice Landy, National Institute of Allergy and Infectious Diseases, Bethesda, Md., for his helpful discussion and advice during the course of this study, as well as his excellent assistance in the preparation of this manuscript.