Comparative Viral Sensitivity of Bovine and Avian Cells Before and After Storage in Liquid Nitrogen.

Abstract

Satisfactory mono-layer cultures were produced after freezing bovine embryonic kidney (BEK) and chicken embryo fibroblast cells in the presence of 7.5 per cent DMSO [dimethyl sulfoxide] in the storage medium and storage for varying lengths of time in liquid nitrogen vapor. The sensitivity of such cultures to selected viruses remained undiminished after 6 mo. of such storage. BEK cells frozen after initial growth as primary monolayer cultures generally provided satisfactory monolayer cultures within a relatively short time (2 to 4 days) as compared with BEK cells which were frozen after fresh procurement from tissues (5 to 8 days).