Trehalase in Conidia of Aspergillus oryzae
- 30 April 1966
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 91 (5), 1883-+
- https://doi.org/10.1128/jb.91.5.1883-1887.1966
Abstract
Trehalases (soluble trehalase and coat-bound trehalase) were found in the conidia of Aspergillus oryzae, and the total activity of the trehalases increased during the germination process. The soluble trehalase was purified by di-ethylaminoethyl-cellulose column chromatography; its optimal pH, Michaelis constant, and heat stability were studied. In vitro, the trehalases were competitively inhibited by D-mannitol, which was also contained in the conidia. Since the trehalose content in the conidia decreased at an early stage of germination, it was assumed that trehalase might begin to hydrolyze trehalose after the inhibitory effect of D-mannitol decreased.This publication has 17 references indexed in Scilit:
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