Acetylcholine Receptor Antibody in Myasthenia Gravis: Purification and Characterization
- 1 December 1978
- journal article
- research article
- Published by Wiley in Scandinavian Journal of Immunology
- Vol. 8 (6), 525-533
- https://doi.org/10.1111/j.1365-3083.1978.tb00552.x
Abstract
Acetylcholine receptor antibody was measured in serum and IgG fractions from patients with myasthenia gravis using different analyses for antibody interfering with the toxin binding site and for antibody directed against othersites. No correlation was found between the concentration of receptor antibody as measured with the two assays. The different antibody activities also showed different isoelectric spectra. Receptor antibody was purified about 1000 times from IgG by affinity chromatography on a partially purified human skeletal muscle receptor preparation. The purified antibody was homogeneous in SDS-gel electrophoresis and showed a polyclonal pattern in agarose gel electrophoresis and isoelectric focusing. Both kinds of light chains were demonstrated, The results imply that the acetytcholine receptor antibody found in myasthenia gravis is heterogeneous and of multiclonal origin.This publication has 27 references indexed in Scilit:
- Determination of acetylcholine receptor antibody in myasthenia gravis: clinical usefulness and pathogenetic implications.Journal of Neurology, Neurosurgery & Psychiatry, 1978
- An assay for antibodies to human acetylcholine receptor in serum from patients with myasthenia gravisClinical Immunology and Immunopathology, 1977
- EFFECTS OF NORMAL AND MYASTHENIC SERUM FACTORS ON INNERVATED AND CHRONICALLY DENERVATED MAMMALIAN MUSCLESAnnals of the New York Academy of Sciences, 1976
- Antibodies Against Nicotinic Acetylcholine Receptor and Skeletal Muscle in Human and Experimental Myasthenia GravisScandinavian Journal of Immunology, 1975
- Antibodies Against Nicotinic Acetylcholine Receptor and Skeletal Muscle in Human and Experimental Myasthenia GravisScandinavian Journal of Immunology, 1975
- Isolation of IgG3 from Normal Human Sera and from a Patient with Multiple Myeloma by Using Protein A‐Sepharose 4BScandinavian Journal of Immunology, 1975
- Immunoglobulin classes and IgG subclasses of human antinuclear antibodies: A correlation of complement fixation and the nephritis of systemic lupus erythematosusClinical Immunology and Immunopathology, 1973
- The Effect of Thoracic Duct Lymph Drainage in Myasthenia gravisEuropean Neurology, 1973
- Antibody isoelectric spectra. Analysis of the heterogeneity of antibody molecules in serum by isoelectric focusing in gel and specific detection with haptenEuropean Journal of Immunology, 1971
- Preparation of Iodine-131 Labelled Human Growth Hormone of High Specific ActivityNature, 1962