Renal ornithine decarboxylase activity, polyamines, and compensatory renal hypertrophy in the rat
- 1 August 1988
- journal article
- research article
- Published by American Physiological Society in American Journal of Physiology-Renal Physiology
- Vol. 255 (2), F270-F277
- https://doi.org/10.1152/ajprenal.1988.255.2.f270
Abstract
We determined the role of ornithine decarboxylase (ODC) in compensatory renal hypertrophy (CRH) by relating renal ODC activity and polyamine content to kidney size, expressed as a percent of body weight, 1 wk after unilateral nephrectomy (UN). In normal rats, renal ODC activity increased from 11.0 +/- 7.4 (SD) to 36.7 +/- 15.4 pmol 14CO2.min-1.g wet wt-1 3 h after UN (P less than 0.002); 1 wk later the remaining kidney weight had increased from 0.38 to 0.46% body weight (P less than 0.001). Renal concentration of putrescine, the product of ODC's decarboxylation of ornithine, was increased 3, 8, and 48 h after UN, but concentrations of polyamines synthesized later in the pathway, spermidine and spermine, were not appreciably affected. Pretreatment with difluoromethylornithine (DFMO), an irreversible inhibitor of ODC, as a 1% drinking water solution inhibited both base-line renal ODC activity and putrescine concentration as well as increases stimulated by UN, although concentrations of spermidine and spermine were not decreased. In these rats, CRH still occurred, since kidney weight increased from 0.36 to 0.46% (P less than 0.001). A 2% DFMO solution caused depletion of all three polyamines, but CRH took place nevertheless. In hypophysectomized rats, both increased renal ODC activity and CRH occurred as well, indicating that these two consequences of UN do not require intact pituitary function. Although increased renal ODC activity and CRH after UN are correlated in normal and hypophysectomized rats, CRH takes place in rats treated with DFMO despite inhibition of ODC activity and depletion of polyamines.(ABSTRACT TRUNCATED AT 250 WORDS)This publication has 24 references indexed in Scilit:
- Polyamines in rapid growth and cancerBiochimica et Biophysica Acta (BBA) - Reviews on Cancer, 1978
- Changes in renal cyclic nucleotide content as a possible trigger to the initiation of compensatory renal hypertrophy in ratsThe Journal of Physiology, 1977
- Proposed model of major sequential biochemical events of a trophic responseLife Sciences, 1976
- Cellular hypertrophy and renal function during compensatory renal growthAmerican Journal of Physiology-Legacy Content, 1976
- In VivoHormonal Induction of Ornithine Decarboxylase in Rat KidneyEndocrinology, 1976
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976
- Accretion and turnover of RNA in the renoprival kidneyAmerican Journal of Physiology-Legacy Content, 1968
- CHEMICAL ASPECTS OF COMPENSATORY RENAL HYPERTROPHY1965
- THE EFFECT OF HYPOPHYSECTOMY, ADRENALECTOMY AND ACTH ADMINISTRATION ON COMPENSATORY RENAL HYPERTROPHY IN RATSQuarterly Journal of Experimental Physiology and Cognate Medical Sciences, 1963
- ENHANCING EFFECTS OF GROWTH HORMONE ON RENAL FUNCTIONAmerican Journal of Physiology-Legacy Content, 1949