Discontinuous buffer systems optimized for the agarose gel electrophoresis of subcellular particles
- 1 January 1988
- journal article
- research article
- Published by Wiley in Electrophoresis
- Vol. 9 (4), 167-171
- https://doi.org/10.1002/elps.1150090404
Abstract
Discontinuous buffer systems operative between pH 5.7 and 7.4, 0 °C, were generated, which are characterized by more rapidly displaced moving boundaries than applied previously. These allow one to resolve subcellular particles relatively rapidly and at relatively low agarose gel concentrations. A commercial mixture of DNA restriction fragments pre‐stained with ethidium bromide was found to be a suitable tracking dye for these boundaries.This publication has 16 references indexed in Scilit:
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