Nephelometric Studies of the Precipitin Reaction: A Model System for Specific Protein Measurements

Abstract

Light-scattering studies have been carried out on an immunochemical system that serves as a model for specific protein determinations in biological samples. The influence of antigen/antibody ratio, salts, urea, pH, temperature, and excitation wavelength are presented. Of significance is the finding that ions, particularly F^-, increase the rate of formation of and the light scattering from the antigen-antibody complexes.