Matrix‐free desorption of biomolecules using massive cluster impact
- 1 December 1994
- journal article
- research article
- Published by Wiley in Rapid Communications in Mass Spectrometry
- Vol. 8 (12), 996-1000
- https://doi.org/10.1002/rcm.1290081218
Abstract
Massive-cluster impact (MCI) ionization was found to be capable of producing high secondary-ion yields from biological samples without the use of a liquid matrix. Dry samples of molecules as large as cytochrome c were observed to be efficiently desorbed without significant fragmentation by the MCI beam of glycerol clusters. In addition, signals from protonated molecules retained a significant proportion of their initial intensity even after several minutes exposure to the primary beam. It was determined that these remarkable phenomena are not the result of the build-up of a layer of glycerol caused by the cluster bombardment.Keywords
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