Purification, Cloning, and Expression of Ciliary Neurotrophic Factor (CNTF)
- 24 November 1989
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 246 (4933), 1023-1025
- https://doi.org/10.1126/science.2587985
Abstract
Ciliary neurotrophic factor (CNTF) is one of a small number of proteins with neurotrophic activities distinct from nerve growth factor (NGF). CNTF has now been purified and cloned and the primary structure of CNTF from rabbit sciatic nerve has been determined. Biologically active CNTF has been transiently expressed from a rabbit complementary DNA clone. CNTF is a neural effector without significant sequence homologies to any previously reported protein.Keywords
This publication has 26 references indexed in Scilit:
- New roles for PDGF and CNTF in controlling the timing of glial cell differentiation in the optic nerveTrends in Neurosciences, 1989
- Type-2 astrocyte development in rat brain cultures is initiated by a CNTF like protein produced by type-1 astrocytesNeuron, 1988
- Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA PolymeraseScience, 1988
- An examination of ciliary neuronotrophic factors from avian and rodent tissue extracts using a blot and culture techniqueDevelopmental Brain Research, 1987
- Primary structure of bovine brain acidic fibroblast growth factor (FGF)Biochemical and Biophysical Research Communications, 1985
- Human β-nerve growth factor gene sequence highly homologous to that of mouseNature, 1983
- Isolation and nucleotide sequence of a cDNA encoding the precursor of mouse nerve growth factorNature, 1983
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- The Genome of Simian Virus 40Science, 1978
- Ribonucleic acid isolated by cesium chloride centrifugationBiochemistry, 1974