Abstract
RNA from tissues subjected to very high doses of 17[beta]-estradiol in vivo and in vitro was injected into the uteri of ovariectomized mice before and after ether washing of the RNA. Alkaline phosphatase content of the atrophied uterus was measured after the RNA injections. Results indicate that alkaline phosphatase induction is due to RNA and that ether washing completely eliminates hormonal contamination. The role played by the hormone-cytoplasm requires further attention.