Dose-Dependent Effects in the Subacute Response of the Rat Lung to Quartz. I. The Cellular Response and the Activity of Lactate Dehydrogenase in the Airways

Abstract

The response of the rat lung to a range of doses of quartz at 50 and 100 days after its administration by intratracheal instillation was investigated by bronchopulmonary lavage. The effects on the numbers of polymorphonulcear leukocytes, lymphocytes and macrophages recovered are described. The size distribution of the recovered macrophages was followed and measurements of total numbers of macrophages in the lung were made using a radioactive tracer technique. The activity of the cytoplasmic enzyme, lactate dehydrogenase, in lavage fluid supernatants was recorded. There was a dose-dependent increase in the recovery of all 3 cell types and an increase in the total numbers of macrophages in the lungs which showed no signs of resolution over the 100 day period studied. Increasing levels of lactate dehydrogenase were also dose-dependent and well correlated with the numbers of polymorphs and lymphocytes recovered. The importance of this prolonged inflammatory reaction in relation to the fibrogenic action of quartz is discussed.