Interaction of GTP‐binding protein Gq with photoactivated rhodopsin in the photoreceptor membranes of crayfish
- 13 September 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 330 (2), 197-200
- https://doi.org/10.1016/0014-5793(93)80272-v
Abstract
Interaction of G-protein with photoactivated rhodopsin (Rh∗) in crayfish photoreceptor membranes was investigated by immunoprecipitation using an antibody against rhodopsin. Two kinds of protein were co-precipitated with rhodopsin. One is an α subunit of class-q G-protein (42 kDa, CGqα) which showed light-induced, dose-dependent binding to rhodopsin, and the other is an actin-like protein (44 kDa) with light-independent binding. Most of the CGqα was available for binding to Rh∗ but was dissociated from Rh∗ in the presence of GTPγS. These findings demonstrate that, in the crayfish photoreceptor, a Gq class of G-protein is activated by Rh∗Keywords
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