CHEMICAL CHARACTERIZATION OF ISOLATED EPIDERMAL DESMOSOMES
Open Access
- 1 November 1974
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 63 (2), 524-530
- https://doi.org/10.1083/jcb.63.2.524
Abstract
Desmosomes, isolated from cow nose epidermis by a method utilizing citrate buffer pH 2.6 and density gradient centrifugation, have been analyzed and found to contain approximately 76% protein, 17% carbohydrate, and 10% lipid. Nonpolar amino acids predominate in desmosomal protein, representing 456 residues per 1,000. The sialic acid content is 5 nM/mg of protein. The lipid fraction is composed of approximately 40% cholesterol and 60% phospholipids.Keywords
This publication has 17 references indexed in Scilit:
- Ruthenium red and violet. II. Fine structural localization in animal tissuesThe Anatomical Record, 1971
- Plasma membrane protein subunit composition. A comparative study by discontinuous electrophoresis in sodium dodecyl sulfate.1971
- Glycoproteins of cell surfaces. A comparative study of three different cell surfaces of the rat.1971
- Electrophoretic analysis of the major polypeptides of the human erythrocyte membraneBiochemistry, 1971
- Morphological and Histochemical Aspects of Glycoproteins at the Surface of Animal CellsInternational Review of Cytology, 1971
- The Reliability of Molecular Weight Determinations by Dodecyl Sulfate-Polyacrylamide Gel ElectrophoresisJournal of Biological Chemistry, 1969
- Photodensitometry in the thin-layer chromatographic analysis of neutral lipidsJournal of Chromatography A, 1968
- Urea extraction of Z bands, intercalated disks, and desmosomesJournal of Ultrastructure Research, 1968
- THE LOCALIZATION OF Mg-Na-K-ACTIVATED ADENOSINE TRIPHOSPHATASE ON RED CELL GHOST MEMBRANESThe Journal of cell biology, 1967
- FINE STRUCTURE OF DESMOSOMES, HEMIDESMOSOMES, AND AN ADEPIDERMAL GLOBULAR LAYER IN DEVELOPING NEWT EPIDERMISThe Journal of cell biology, 1966