Molecular Vehicle Properties of the Broad Host Range Plasmid RK2
- 19 December 1975
- journal article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 190 (4220), 1226-1228
- https://doi.org/10.1126/science.1060178
Abstract
The pladmid RK2 is stably maintained in a broad range of gram-negative bacteria. The RK2 DNA has a single Eco RI restriction site. The insertion of a DNA fragment into this site does not interfere with either plasmid maintenance or self-transmissibility. Because RK2 has a broad host range, it should be useful for the construction in vitro of hybrid plasmid molecules capable of being established by conjugal transfer or transformation into many genera of gram-negative organisms.Keywords
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