Invertase biosynthesis by Saccharomyces carlsbergensis in batch and continuous cultures
- 31 July 1976
- journal article
- research article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 18 (8), 1103-1115
- https://doi.org/10.1002/bit.260180807
Abstract
The biosynthesis of invertase by Saccharomyces carlsbergensis LAM 1068 was studied in relation to its glucose effect at both unsteady and steady states of growth. Experimental correlations between the dilution rate and invertase specific activity (E/X) in chemostat, cultures led to an optimum for the enzyme synthesis at a particular intermediate growth rate. The value of E/X increased from 1.1 (U/mg biomass) in batch cultures to 13 (U/mg biomass) in chemostat cultures. A mutant strain A3 showed the highest value for E/X = 25 (U/mg biomass) at high dilution rates where glucose repression was observed with the wild strain.This publication has 8 references indexed in Scilit:
- The effects of glucose and oxygen on the cytochromes and metabolic activity of yeast batch cultures. I.Saccharomyces spp.Biotechnology & Bioengineering, 1971
- Effect of growth rate and substrate limitation on the composition and structure of the cell wall of Saccharomyces cerevisiaeBiochemical Journal, 1967
- The Crabtree Effect: A Regulatory System in YeastJournal of General Microbiology, 1966
- Factors influencing the appearance of invertase in Saccharomyces cerevisiaeArchives of Biochemistry and Biophysics, 1964
- Some observations on the form and location of invertase in the yeast cellBiochemical Journal, 1961
- Invertase Formation in Saccharomyces fragilisJournal of General Microbiology, 1956
- Ester formation by yeasts. 1. Ethyl acetate formation by Hansenula speciesBiochemical Journal, 1951
- An application of the method of Hagedorn and Jensen to the determination of larger quantities of reducing sugarsBiochemical Journal, 1929