Eimeria tenella: quantitative in vitro and in vivo studies on the effects of mouse polyclonal and monoclonal antibodies on sporozoites
- 1 September 1986
- journal article
- research article
- Published by Wiley in Parasite Immunology
- Vol. 8 (5), 467-480
- https://doi.org/10.1111/j.1365-3024.1986.tb00862.x
Abstract
Summary Murine, polyclonal and monoclonal antibodies, raised against sporozoites of Eimeria tenella, were tested for their ability to neutralize sporozoite infectivity in vitro and in vivo. Neutralization was effected via three mechanisms. Firstly, sporozoites fixed complement, at low titres, and lysis occurred by the alternative pathway of complement activation. Secondly, in the absence of complement activity, the murine heat-inactivated, hyperimmune antiserum neutralized sporozoites at relatively low titres. At high titres, even though sporozoites were agglutinated, neither the heat-inactivated hyperimmune antiserum nor the monoclonal antibody neutralized sporozoites. Finally, in the presence of complement and specific antibodies, at titres which by themselves would not neutralize sporozoites, neutralization was effected due to lysis via the classical pathway of complement activation.Keywords
This publication has 26 references indexed in Scilit:
- Eimeria tenella (Eucoccidiorida): A Quantitative Assay for Sporozoite Infectivity In Vivo1The Journal of Protozoology, 1986
- Purification of Eimeria Sporozoites by DE‐52 Anion Exchange ChromatographyThe Journal of Protozoology, 1984
- Eimeria tenella: in vitro development in irradiated bovine kidney cellsParasitology, 1984
- Ultrastructural Localization of Monoclonal IgG Antibodies for Antigenic Sites of Eimeria tenella Oocysts, Sporocysts, and Sporozoites1The Journal of Protozoology, 1983
- Complement mediated lysis in chickensDevelopmental & Comparative Immunology, 1982
- Surface Changes Induced by Immune Serum on Eimeria tenella Sporozoites and MerozoitesThe Journal of Protozoology, 1982
- An Electron Microscopical Study of the Interaction of Monoclonal Antibodies with Gametes of the Malarial Parasite Plasmodium gallinaceum1The Journal of Protozoology, 1981
- Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-SepharoseImmunochemistry, 1978
- Immunity to coccidiosis: effect of serum antibodies on cell invasion by sporozoites ofEimeria in vitroParasitology, 1972
- Immunity to coccidiosis: Gut permeability changes in response to sporozoite invasionCellular and Molecular Life Sciences, 1969