A Versatile Assay for High-Throughput Gene Expression Profiling on Universal Array Matrices
Open Access
- 3 May 2004
- journal article
- research article
- Published by Cold Spring Harbor Laboratory in Genome Research
- Vol. 14 (5), 878-885
- https://doi.org/10.1101/gr.2167504
Abstract
We report a flexible, sensitive, and quantitative gene-expression profiling system for assaying more than 400 genes, with three probes per gene, for 96 samples in parallel. The cDNA-mediated annealing, selection, extension and ligation (DASL) assay targets specific transcripts, using oligonucleotides containing unique address sequences that can hybridize to universal arrays. Cell-specific gene expression profiles were obtained using this assay for hormone-treated cell lines and laser-capture microdissected cancer tissues. Gene expression profiles derived from this assay were consistent with those determined by qRT-PCR. The DASL assay has been automated for use with a bead-based 96-array matrix system. The combined high-throughput assay and readout system is accurate and efficient, and can cost-effectively profile the expression of hundreds of genes in thousands of samples.Keywords
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