The reactivity of Reed–Sternberg cells with monoclonal antisera at thin section and ultrastructural levels

Abstract

In an attempt to improve the morphology of Reed–Sternberg cells after immunochemical procedures, the biotin–avidin technique has been used. Satisfactory morphology was obtained at the level of both light and electron microscopy. Reed–Sternberg cells failed to react with a monoclonal antimonocyte serum but gave positive results with anti-1a and FMC7, a monoclonal anti-B cell serum. Positive results were also obtained with FMC 1 and B 1 both of which are monoclonal B cell antisera.