Long-Term Mixed Cultures of Human Hemic Cells, with Granulocytic, Lymphocytic, Plasmocytic and Erythrocytic Series Represented

Abstract

New culture strains containing humman hemic cells of monocytic, lymphocytic, granulocytic, plasmocytic and erythrocytic series are described with suggestions as to maintenance of the desired cell series in culture. The addition of cells from lymphocytic, plasmocytic and erythrocytic series to a previously well established culture has made possible long-term culture of these cell types, since this procedure aids in maintaining the necessary high gradient factor for their growth and multiplication. Although the life span of these cells has been considerably shortened in culture and immature forms predominate, the cells show recognizable characteristics of the cell series of origin. A high population density must be maintained in cultivation of the mixed cultures or the desired cell type may be lost.