Heparan sulfates from Swiss mouse 3T3 and SV3T3 cells: O-sulfate difference

Abstract

A difference in the extent of sulfation between the heparan sulfate isolated from Swiss 3T3 mouse fibroblasts and that from Swiss 3T3 cells transformed by the DNA virus SV40 was reported previously. This variance is manifested by different chromatographic and electrophoretic properties. Heparan sulfates from the 2 cell types were treated with nitrous acid under conditions that gave selective deaminative cleavage of glucosaminyl residues with sulfated amino groups in order to define the nature of the difference in sulfation further. The O-sulfate-containing fragments from the heparan sulfates were compared by gel filtration and ion-exchange chromatography. The 3T3 heparan sulfate contains 8% more O-sulfate than does the SV3T3 heparan sulfate. Analysis of uronic acids revealed that both types of heparan sulfates contain 45% L-iduronic acid and 55% D-glucuronic acid. These and other observations indicate that the primary difference in sulfation between the 3T3 and SV3T3 heparin sulfates lies in the extent of O-sulfation.