A Method for Direct Demonstration of Pleuropneumonia-Like Organisms in Cultured Cells.
- 1 December 1964
- journal article
- research article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 117 (3), 899-901
- https://doi.org/10.3181/00379727-117-29731
Abstract
Summary Direct microscopic observation of pleuropneumonia-like organisms (PPLO) in cell cultures is easily accomplished following hypotonic treatment, air-drying and staining with orcein. A rapid technique using FL human amnion cells, inoculated with supernatant from suspected cultures, is described. The demonstration of PPLO contamination of 30 cell lines by this rapid method was in complete agreement with results of PPLO-agar techniques.Keywords
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- CHROMOSOMES OF PRIMARY HUMAN AMNION CELLS AND FL CELLS1963
- PLEUROPNEUMONIA-LIKE (MYCOPLASMA) INFECTIONS OF TISSUE CULTUREJournal of Bacteriology, 1961
- FINE STRUCTURE OF PLEUROPNEUMONIALIKE ORGANISMS IN PURE CULTURE AND IN INFECTED TISSUE CULTURE CELLSJournal of Bacteriology, 1960