Metal-dependent folding of a single zinc finger from transcription factor IIIA.
- 1 July 1987
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 84 (14), 4841-4845
- https://doi.org/10.1073/pnas.84.14.4841
Abstract
A 30-amino acid peptide, which corresponds to the second "zinc finger" domain of transcription factor IIIA, has been synthesized and purified. This peptide folds in the presence of zinc: adding Zn2+ significantly changes the circular dichroism spectrum, and Zn2+ protects the peptide from tryptic digestion. The peptide also binds Co2+, and the absorption spectrum of the Co2+ complex suggests that a tetrahedral binding site is formed by two cysteines and two histidines. Experiments at higher temperatures (60-75.degree. C) suggest that these folded metal-peptide complexes are quite thermostable. The peptide shows some sequence-specific effects in DNase and methylation protection experiments. However, it does not give a clear "footprint," and some effects are observed in the absence of added zinc.Keywords
This publication has 28 references indexed in Scilit:
- Structure of the DNA-Eco RI Endonuclease Recognition Complex at 3 Å ResolutionScience, 1986
- Sequence homology of the yeast regulatory protein ADR1 with Xenopus transcription factor TFIIIANature, 1986
- Sequence and structure of the Serendipity locus of Drosophila melanogasterJournal of Molecular Biology, 1985
- The primary structure of transcription factor TFIIIA has 12 consecutive repeatsFEBS Letters, 1985
- Domains of the positive transcription factor specific for the Xenopus 5S RNA geneCell, 1984
- The binding of a transcription factor to deletion mutants of a 5S ribosomal RNA geneCell, 1981
- Specific interaction of a purified transcription factor with an internal control region of 5S RNA genesCell, 1980
- A control region in the center of the 5S RNA gene directs specific initiation of transcription: II. The 3′ border of the regionCell, 1980
- A control region in the center of the 5S RNA gene directs specific initiation of transcription: I. The 5′ border of the regionCell, 1980
- Calcium-dependent bacteriophage DNA infectionJournal of Molecular Biology, 1970