Dip-Pen Nanolithography of Reactive Alkoxysilanes on Glass
- 11 September 2003
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 125 (40), 12096-12097
- https://doi.org/10.1021/ja0363720
Abstract
The use of organofunctional silane chemistry is a flexible and general method for immobilizing biomolecules on silicon oxide surfaces, including fabricating DNA, small-molecule, and protein microarrays. The biggest hurdle in employing dip-pen nanolithography (DPN) for extending this general approach to the nanoscopic domain is the tendency of trialkoxy- and trichlorosilanes to rapidly polymerize due to hydrolysis reactions. The control of the local water concentration between the substrate surface and the scanning AFM tip is critical, both to the physical and chemical processes involved in DPN writing and to the ability to form well-defined thin layers of reactive silanes without extensive polymerization induced disorder. We found that we could control the degree of polymerization through careful choice of the alkoxysilane used as the “ink” for DPN and through control of the relative humidity during inking and writing with the coated AFM tip. As a proof-of-principle, we demonstrate that areas patterned with an alkoxysilane on glass with DPN are functional for subsequent immobilization of fluorescently labeled streptavidin via covalent attachment of biotin. This preliminary result sets the stage for the ability to capture proteins in their fully hydrated state from buffered solution, by molecular recognition onto previously written reactive nanoscopic regions on oxidized silicon and glass.Keywords
This publication has 19 references indexed in Scilit:
- Anomalous Surface Diffusion in Nanoscale Direct Deposition ProcessesPhysical Review Letters, 2003
- Molecular Recognition-Mediated Fabrication of Protein Nanostructures by Dip-Pen LithographyNano Letters, 2002
- Creating Nanoscale Patterns of Dendrimers on Silicon Surfaces with Dip-Pen NanolithographyNano Letters, 2002
- How to Make a DNA ChipAngewandte Chemie International Edition, 2002
- Thiol Diffusion and the Role of Humidity in “Dip Pen Nanolithography”Physical Review Letters, 2002
- Surface Chemistry of Mercaptan and Growth of Pyridine Short-Chain Alkoxy Silane Molecular LayersLangmuir, 2002
- "Dip-Pen" NanolithographyScience, 1999
- Functionalized Monolayers on Ordered Mesoporous SupportsScience, 1997
- Surface reactions on thin layers of silane coupling agentsLangmuir, 1993
- The Evolution of Silicon Wafer Cleaning TechnologyJournal of the Electrochemical Society, 1990