SOME BIOLOGICAL PROPERTIES OF BACTERIOPHAGES S13 AND φX-174
- 1 March 1958
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 75 (3), 310-315
- https://doi.org/10.1128/jb.75.3.310-315.1958
Abstract
The two phages are serologically related, but not identical. They differ in ion requirements for plaque formation on three different hosts. On Shigella paradysenteriae strain Y6R, S13 has a latent period of 14 to 15 minutes and a burst size of 200 to 250 under optimal conditions. A number of host-range and plaque-type mutants of S13 are described. Cells infected simultaneously with two different mutants release both types among the phage progeny. Less than one progeny phage per 1000, if any, is a recombinant type. Multiplicity reactivation of UV-killed S13 could not be shown. UV-killed S13 particles do not kill host cells. Photoreactivation was demonstrated. Methods for producing high-titer lysates are given.Keywords
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