A comparison of a fluorescent antibody technique with a cultural method in the detection of infections with Shigella sonnei

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Abstract
A comparison has been made of a fluorescent antibody technique with a cultural method for the detection of Sh. sonnei in faeces. The two methods were in agreement in 73% of the 394 specimens examined; 57 (14-5%) specimens were positive by culture only; 52 (13.2%) specimens gave positive results by fluorescence microscopy which were not confirmed by culture. Most of the latter are thought to be "false positive" results. The value and usefulness of fluorescence microscopy in the diagnosis of Sonne dysentery is discussed.