Studies on peptides. CXL Synthesis of human gastrin-releasing polypeptide (hGRP).
- 1 January 1986
- journal article
- research article
- Published by Pharmaceutical Society of Japan in CHEMICAL & PHARMACEUTICAL BULLETIN
- Vol. 34 (6), 2462-2470
- https://doi.org/10.1248/cpb.34.2462
Abstract
Human gastrin-releasing polypeptide (hGRP), which consists of 27 amino acid residues with a C-terminal amide, was synthesized by a conventional solution method, by assembling six peptide fragments followed by deprotection with 1 M trifluoromethanesulfonic acid-thioanisole in trifluoroacetic acid. Met(O) employed was reduced by treatment with phenylthiotrimethylsilane in the presence of trimethylsilyl trifluoromethanesulfonate before deprotection. The synthetic peptide was as active as synthetic porcine GRP, in terms of immunoreactive gastrin release in rats.This publication has 12 references indexed in Scilit:
- Cloning and characterization of cDNAs encoding human gastrin-releasing peptide.Proceedings of the National Academy of Sciences, 1984
- Isolation and sequence analysis of human bombesin-like peptidesPeptides, 1984
- Neuromedin C: A bombesin-like peptide identified in porcine spinal cordBiochemical and Biophysical Research Communications, 1984
- Studies on peptides. CXXI. Nin-Mesitylenesulfonyltryptophan, a new derivative for peptide synthesis.CHEMICAL & PHARMACEUTICAL BULLETIN, 1984
- Amino acid sequences of three bombesin-like peptides from canine intestine extracts.Journal of Biological Chemistry, 1983
- STUDIES ON PEPTIDES .111. SYNTHESIS OF CHICKEN GASTRIN-RELEASING PEPTIDE1982
- Studies on peptides. 103. Chemical synthesis of a crystalline protein with the full enzymic activity of ribonuclease AJournal of the American Chemical Society, 1981
- STUDIES ON PEPTIDES .102. SYNTHESIS OF A HEPTACOSAPEPTIDE AMIDE CORRESPONDING TO THE ENTIRE AMINO-ACID-SEQUENCE OF GASTRIN-RELEASING PEPTIDE1981
- Total synthesis of bovine pancreatic ribonuclease A. Part 1. Synthesis of the protected pentadecapeptide ester (positions 110–124)Journal of the Chemical Society, Perkin Transactions 1, 1981
- Characterization of a gastrin releasing peptide from porcine non-antral gastric tissueBiochemical and Biophysical Research Communications, 1979