TRACER STUDIES ON ORNITHINE, LYSINE, AND FORMATE METABOLISM IN AN AMINO ACID FERMENTING CLOSTRIDIUM

Abstract
A clostridium, strain HF, decomposes 2-C14ornithine chiefly by an oxidative pathway to NH3, CO2, and 1-C14acetate. A small amt. of 1,3-C14 -butyrate is formed. Only about 6% of the ornithine is reduced to delta-amino valeric acid. 2-C14 lysine is converted to NH3, 2-C14 acetate, and butyrate which is labeled predominantly in carbon 2. In the presence of sulfide, but not thioglycolate, C14 formate is converted to doubly-labeled acetate.