Bovine Brain S100 Proteins: Separation and Characterization of a New S100 Protein Species
- 31 December 1982
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 40 (1), 145-152
- https://doi.org/10.1111/j.1471-4159.1983.tb12664.x
Abstract
Three S100 protein species (S100a, S100b, S100a'') were purified from bovine brain using a modification of standard preparative methods. A higher yield for each protein was obtained at the last separation step. Characterization by urea/sodium dodecyl sulfate/polyacrylamide gel electrophoresis, UV absorption spectra and fluorescence parameters, provided evidence of a new tryptophan-containing S100 protein called S100a'', which exhibits, as S100a and S100b, the properties of a Ca2+ binding protein.Keywords
This publication has 26 references indexed in Scilit:
- Specific and Artefactual Cellular Localizations of S100 Protein: An Astrocyte Marker in Rat CerebellumDevelopmental Neuroscience, 1981
- HETEROGENEITY OF S‐100 PROTEINS OF BRAIN: SUBUNIT COMPOSITIONSJournal of Neurochemistry, 1979
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- INTRINSIC FLUORESCENCE OF S4 AND S7 E. COLI RIBOSOMAL PROTEINS*Photochemistry and Photobiology, 1975
- Interaction of S-100 protein with cations and liposomesBiochemistry, 1974
- Étude de la fraction protéique neurospecifique S-100 : I. Présence in vivo des composants rapide et lent de la fraction protéique S-100Biochimie, 1971
- Effect of calcium ion on S-100, a protein of the nervous systemBiochemistry, 1969
- A soluble protein characteristic of the nervous systemBiochemical and Biophysical Research Communications, 1965
- DISC ELECTROPHORESIS – II METHOD AND APPLICATION TO HUMAN SERUM PROTEINS*Annals of the New York Academy of Sciences, 1964
- DISC ELECTROPHORESIS‐I BACKGROUND AND THEORY*Annals of the New York Academy of Sciences, 1964