ON THE VELOCITY OF OXYGEN DISSOCIATION OF HUMAN HEMOGLOBIN AND RED CELL

Abstract

The deoxygenation velocities of Hb hemoglobin solution and the red cell suspension were measured at 37 degrees C by mixing the oxygenated Hb and red cells with an O2 free solution. The dissociation of O2Hb in hemolysed solution occurred very fast and the half-time was within 5 msec. Moreover, it was clarified that the rate of change in SO2 [O2 saturation] of Hb solution obeyed the following formula, dS/dt= 63.4 (S [long dash] Sm) 1.6, where Sm is the SO2 corresponding to Po, of the surrounding medium. In the red cell suspension, the reaction proceeded comparatively slowly and the half-times were ranged from 35 to 65 msec. The rate of SQ2 change was expressed by use of the velocity factor, F[image], as follows: dS/dt=F[image] (Pc[long dash]Pm)/0.4X10-2, where Pc and Pm are the Po2, m the red cell and the medium respectively. Fc seemed to be fairly constant and it did not deviate out of the range of 0.02 to 0.03 sec-1 mmHg-1.