AMINO ACID INCORPORATION IN PIGEON PANCREAS FRACTIONS

Abstract

In the study of cell-free preparations of pigeon pancreas, amino acid incorporation into microsome protein occurs if a properly complex mixture of enzyme proteins and one or several polynucleotides is added. These polynucleo-tides, present in the non-particulate fraction of pigeon pancrease, are heat-stable and non-dialyzable. Amino acid transfer to the polynucleo-tide carrier can be shown on incubation with dialyzed supernate. The authors conclude that the polynucleotide component acts as an intermediary between activating enzymes and the microsomes.