Characterization of an RNA-directed DNA polymerase found in association with murine intracytoplasmic A-particles
- 1 May 1977
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 22 (2), 273-280
- https://doi.org/10.1128/jvi.22.2.273-280.1977
Abstract
An RNA-directed DNA polymerase was associated with intracytoplasmic A-particles from DBA/2 mouse leukemia cells. The enzyme activity was detected after disrupting the purified particles with 2 M NaCl-20 mM dithiothreitol. The presence of a divalent cation and all 4 deoxyribonucleoside triphosphates was essential for this enzyme activity. The enzyme had a clear preference for Mg2+ over Mn2+. Cs2SO4 isopycnic gradient centrifugation of the DNA product synthesized in the actinomycin D-containing reaction revealed the presence of DNA-RNA hybrid. The purified DNA product hybridized with RNA isolated from A-particles. The endogenous A-particle RNA apparently serves as the template for the DNA polymerase.This publication has 28 references indexed in Scilit:
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