Chemotaction of Mouse Spermatozoa Induced by Certain Hormones

Abstract

Chemotaxis assays of mouse spermatozoa were performed in vitro. Amounts of calcitonin (5.0 IU/ml, 10.0 IU/mL) and acetylcholine (1.0 mg/ml) in Biggers-Whitter-Whittingham medium filled out wells of experimental plate were increased directly by migration of mouse spermatozoa to the medium containing these hormones. This effect was interpreted as chemotaxis of spermatozoa. Low concentrations of hormones were not attractants and high concentrations of acetylcholine (5.0 mg/ mL) decreased spermatozoa migration. Glucagon and vasopressin results in a decrease in concentration of migrated spermatozoa. In low concentrations of these hormones differences in sperm migration were not observed. Presence of histamine and thyroxine in BWW medium did not affect the migration behavior of mouse spermatozoa in vitro.