Electrical properties and structure of the frog arachnoid membrane
- 30 April 1976
- journal article
- research article
- Published by Wiley in Journal of Neurobiology
- Vol. 7 (3), 259-270
- https://doi.org/10.1002/neu.480070309
Abstract
We have used an in vitro preparation of the frog arachnoid membrane to study the role of this membrane in the maintenance of the “blood‐cerebrospinal fluid (csf) barrier.” Electron microscopy showed that the membrane was made up of 10–15 layers of flat epithelial cells joined together by numerous cell junctions. The electrical resistance of the preparation was about 2000 ohms cm2. The steady‐state transmural potential difference (pd) ranged up to 45 mV, csf positive, and this was eliminated by either the addition of ouabain to the csf, or by replacing the NaCl with TEA Cl. The pd across the membrane increased when bicarbonate was added to the external bathing solutions. We conclude that this pd is due to the active transport of sodium from the subural fluid to the csf. In some preparations the transmural pd was reversed, i.e., csf negative, and this was also abolished by the addition of ouabain to the csf, or by replacing chloride with isethionate. We conclude that this pd is related to active chloride transport. These, and other experiments, lead us to the conclusion that the arachnoid membrane is involved in the production of the cerebrospinal fluid and the maintenance of the blood‐cerebrospinal fluid barrier.This publication has 28 references indexed in Scilit:
- The effects of electrical and osmotic gradients on lateral intercellular spaces and membrane conductance in a low resistance epitheliumThe Journal of Membrane Biology, 1974
- Electrically and osmotically induced changes in permeability and structure of toad urinary bladderBiochimica et Biophysica Acta (BBA) - Biomembranes, 1974
- Function of the thick ascending limb of Henle's loopAmerican Journal of Physiology-Legacy Content, 1973
- Mechanisms of ion transport across the choroid plexusThe Journal of Physiology, 1972
- The mechanism of cation permeation in rabbit gallbladderThe Journal of Membrane Biology, 1971
- Junction potentials, electrode standard potentials, and other problems in interpreting electrical properties of membranesThe Journal of Membrane Biology, 1970
- Ultrastructure of Meningeal SheathsArchives of Ophthalmology (1950), 1969
- Electroosmosis in Membranes: Effects of Unstirred Layers and Transport Numbers: II. ExperimentalBiophysical Journal, 1969
- Electroosmosis in Membranes: Effects of Unstirred Layers and Transport Numbers: I. TheoryBiophysical Journal, 1969
- A rapid method for determining voltage-concentration relations across membranesThe Journal of Physiology, 1966