Rapid-flow enthalpimetric determination of urea in serum, with use of an immobilized urease reactor.

Abstract

We used a differential thermal detector in conjunction with an immobilized urease reactor to determine urea in serum. Samples (120 mul) are introduced into a flow stream and passed through an "adiabatic" column, which is packed with enough insolubilized urease to completely convert urea to ammonia and carbon dioxide. Measured temperature changes are directly proportional to the serum urea concentration. Urea in the presence of protein, bilirubin, and hemoglobin can thus be rapidly, simply, and inexpensively measured. Results correlate well with those obtained by the manual diacetyl monoxime and urease/indophenol methods.