The construction of single copy promoter-probe vectors for Bacillus subtilis.
- 1 January 1986
- journal article
- research article
- Published by Microbiology Research Foundation in The Journal of General and Applied Microbiology
- Vol. 32 (1), 67-72
- https://doi.org/10.2323/jgam.32.67
Abstract
A series of single copy promoter-probe vectors for Bacillus subtilis was constructed from phage .vphi.105 by introducing a promoterless amylase gene. With these vectors, strong promoters were cloned from Bacillus licheniformis chromosomal DNA. In addition, the transcriptional direction of the kan of pUB110 was determined using the DNA fragments within these phages as promoter-probe cartridges.This publication has 13 references indexed in Scilit:
- Translational block to expression of the Escherichia coli Tn9-derived chloramphenicol-resistance gene in Bacillus subtilis.Proceedings of the National Academy of Sciences, 1982
- Nucleotide sequence of the promoter and NH2-terminal signal peptide region of the α-amylase gene from Bacillus amyloliquefaciensGene, 1981
- Cloning restriction fragments that promote expression of a gene in Bacillus subtilisJournal of Bacteriology, 1981
- Cloning of sporulation gene spoOB of Bacillus subtilis and its genetic and biochemical analysisJournal of Bacteriology, 1981
- Characterization of chimeric plasmid cloning vehicles in Bacillus subtilisJournal of Bacteriology, 1980
- A method for construction of specialized transducing phage ϱ11 of Bacillus subtilisGene, 1979
- High frequency transformation of Bacillus subtilis protoplasts by plasmid DNAMolecular Genetics and Genomics, 1979
- Characterization of Staphylococcus aureus plasmids introduced by transformation into Bacillus subtilisJournal of Bacteriology, 1978
- Bacteriocin and antibiotic resistance plasmids in Bacillus cereus and Bacillus subtilisJournal of Bacteriology, 1978
- Catabolic repression of bacterial sporulation.Proceedings of the National Academy of Sciences, 1965