Cultivation of Poliomyelitis Virus in Cultures of Human Foreskin and Embryonic Tissues.

Abstract

Continuation of previously reported studies on the cultivation of poliomyelitis virus in suspended cell cultures of human embryonic tissues has resulted in the maintenance of the Lansing strain for 224 days through 13 serial cultures in mixed embryonic skin and muscle tissues. In addition, this strain has been maintained for 173 days in 2 lines of 11 serial cultures each and composed, respectively, of human embryonic intestine and brain. New expts. are reported which demonstrate that the Lansing strain of virus will likewise multiply in cultures of human prepuce, thus the agent was maintained through 5 serial passages in this medium for a total period of 88 days. Preliminary results of attempts to cultivate another strain of poliomyelitis virus, the Brunhilde strain, are also presented; this strain has been maintained for 73 days in cultures of human embryonic skin and muscle tissue and for 39 days in human embryonic brain tissue.