The prevalence and necessity for early detection sion problems illustrate the need for improved methods of vision screening in preschool children. This study assessed the validity and reliability of a new device, the MTI Photoscreener in a cross-sectional field study. An appropriate sample size (>140) was calculated and recruited for the study. All children (N = 161) in a migrant workers summer education program were screened with the MTI Photoscreener. Simultaneously and in a masked design, disease status was determined by the odified Clinic Technique, a well established method for diagnosing the conditions which the MTI screener was designed to detect. Validity measures revealed a sensitivity of 54%, specificity of 87%, Ø coefficient of 0.40, and positive predictive value of 52%. Repeatability was assessed by the k coefficient, by a test for effect modification by examiner, and by comparison of sensitivity and specificity across 12 masked examiners. The k coefficient was 0.38. A test for effect modification suggested that differences existed among the examiners. Variability of sensitivity was high, but variability of specificity was low. Methods for vision screening in preschool children are limited. The MTI Photoscreener is an easy and efficient method, but the validity and reliability is a concern. Comparison of our results with other studies suggests future potential for this instrument provided protocols are refined and further field studies reveal efficacy.