Studies on L-Glycerol-3-phosphate Dehydrogenases in Japanese Quail, Coturnix coturnix japonica

Abstract

Multiple components and some enzymatic properties of L-glycerol-3-phosphate: NAD oxidoreductase [EC 1. 1. 1. 8] from liver and testes in Japanese quail, Cotumix coturnix japonica have been investigated. Liver enzyme showed five components with isoelectric points at pH 7.10 (, designated P-I), 7.38 (P-II), 7.54 (P-III), 7.62 (P-IV), and 7.79 (P-V). P-III among these five components had predominant activity. Testes enzyme showed four components with isoelectric points at pH 4.58, 4.90, 5.26, and 5.46. No predominant component was present among them. The apparent K_m values for L-glycerol-3-phosphate of the partially purified liver and testes enzymes were 3.39 him and 0.33 mM, respectively, whereas no significant difference in K_m value for NAD⁺ of both enzymes was observed.