Detection of Monoclonal Antibody-Defined Colorectal Carcinoma Antigen by Solid-Phase Binding Inhibition Radioimmunoassay
- 1 January 1981
- journal article
- Published by Mary Ann Liebert Inc in Hybridoma
- Vol. 1 (1), 37-45
- https://doi.org/10.1089/hyb.1.1981.1.37
Abstract
We have established a solid-phase binding inhibition radioimmunoassay for the detection of colorectal carcinoma-specific antigens in tissue culture supernatants of human colorectal carcinoma cell lines and in serum and urine of colorectal carcinoma patients. Using the [3H]glucosamine-labeled cell membrane glycolipid antigen and colorectal carcinoma-specific monoclonal antibodies in this assay, we have been able to detect several human colorectal carcinoma membrane-specific antigens that are released from the cell membrane into tissue culture supernatants, and an antigen detected by antibodies 1116-NS-19-9 and 1116-NS-52a that is found only in the serum and urine of cancer patients.Keywords
This publication has 7 references indexed in Scilit:
- Specific Antigen in Serum of Patients with Colon CarcinomaScience, 1981
- A Monosialoganglioside Is a Monoclonal Antibody-Defined Antigen of Colon CarcinomaScience, 1981
- Production of monoclonal antibodies in serum free mediumJournal of Immunological Methods, 1980
- Colorectal carcinoma antigens detected by hybridoma antibodiesSomatic Cell and Molecular Genetics, 1979
- Study of antibodies against human melanoma produced by somatic cell hybrids.Proceedings of the National Academy of Sciences, 1978
- Characterization of human malignant melanoma cell linesIn Vitro Cellular & Developmental Biology - Plant, 1977
- Enzymatic Susceptibility and Spontaneous Release of Human Melanoma Tumor-Associated Antigens2JNCI Journal of the National Cancer Institute, 1977