Matrix‐assisted laser desorption/ionization mass spectrometry of nucleic acids with wavelengths in the ultraviolet and infrared
- 1 December 1992
- journal article
- research article
- Published by Wiley in Rapid Communications in Mass Spectrometry
- Vol. 6 (12), 771-776
- https://doi.org/10.1002/rcm.1290061212
Abstract
A number of different matrices have been tested and compared for ultraviolet and ubfrared (UV and IR) matrix-assisted lasedr desorption/ionization (MALDI) of oligodeoxyribonucleotides and mixtures thereof, as well as ribonucleic acids (tRNA from yeast and rRNA from Ecoli). A new technique for removing alkali cations from nucleic acid samples during sample preparation on the sample support is demonstrated. The amount of oligonucleotide sample consumed during a typical measurement in IR-MALDI-MS was determined.Keywords
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