Damage mechanisms of suspended animal cells in agitated bioreactors with and without bubble entrainment
- 20 August 1990
- journal article
- research article
- Published by Wiley in Biotechnology & Bioengineering
- Vol. 36 (5), 476-483
- https://doi.org/10.1002/bit.260360507
Abstract
We show that when freely suspended hybridoma cells are cultured in an agitated bioreactor, two fluid-mechanical mechanisms can cause cell damage and growth retardation. The first is present only when there is a gas phase, and is associated with vortex formation accompanied by bubble entrainment and breakup. In the absence of a vortex and bubble entrainment, cells can be damaged only at very high agitation rates, above approximately 700 rpm, by stresses in the bulk turbulent liquid. Cell damage then correlates with Kolmogorov eddy sizes similar to or smaller than the cell size. In the absence of a vortex, the entrainment and motion of very fine bubbles cause no growth retardation even at agitation rates as high as 600 rpm.This publication has 26 references indexed in Scilit:
- A need for systematic investigations into the material properties of cultured animal cellsTrends in Biotechnology, 1989
- Increasing serum concentrations decrease cell death and allow growth of hybridoma cells at higher agitation ratesBiotechnology Letters, 1989
- Effect of mechanical agitation on hybridoma cell growthBiotechnology Letters, 1988
- Shear Stress Effects on Human T Cell FunctionBiotechnology Progress, 1988
- Experimental evaluation of laminar shear stress on the behaviour of hybridoma mass cell cultures, producing monoclonal antibodies against mitochondrial creatine kinaseJournal of Biotechnology, 1988
- Flow effects on the viability and lysis of suspended mammalian cellsBiotechnology Letters, 1987
- Growth of hybridoma cells under different agitation conditionsBiotechnology Letters, 1986
- Flow Effects on Prostacyclin Production by Cultured Human Endothelial CellsScience, 1985
- Reduced erythrocyte deformability associated with calcium accumulationBiochimica et Biophysica Acta (BBA) - Biomembranes, 1982
- A model system for evaluating shear in the design of stirred fermentorsBiotechnology & Bioengineering, 1966