Determination of Evans'Blue in Plasma and Plasma-Dextran Mixtures By Protein Precipitation and Extraction.
- 1 February 1964
- journal article
- research article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 115 (2), 564-567
- https://doi.org/10.3181/00379727-115-28970
Abstract
Summary A relatively rapid procedure for determination of Evans' blue in plasma or in plasma diluted with dextran is described. The procedure involves the total precipitation of plasma proteins by CdSO4 and NaOH. After centrifugation and acidification of the precipitate it is extracted with butanol. The O.D. of the extract, with ethanol added, is read at 620 mμ, and the dye concentration is read from a standard graph.Keywords
This publication has 4 references indexed in Scilit:
- Blood VolumePhysiological Reviews, 1959
- ESTIMATION OF EVANS BLUE DYE IN BLOOD PLASMA AND ITS APPLICATION TO BLOOD VOLUME DETERMINATION1958
- Some observations on impurities present in samples of Evan's Blue (T 1824) and their influence on blood-volume determinations effected by the dye methodThe Journal of Physiology, 1954
- A MODIFIED DIPHENYLAMINE PROCEDURE FOR THE DETERMINATION OF INULINJournal of Biological Chemistry, 1949