Nonenzymatic methylation of DNA by S-adenosylmethionine in vitro

Abstract

S-Adenosylmethionine was found to methylate DNA non-enzymatically to produce the same putative promutagenic and procarcinogenic lesions formed by carcinogenic chemical methylating agents. The formation of 7-methylguanine was confirmed by u.v. spectrophotometry, the formation of O⁶-methylguanine and 3-methyladenine was suggested by the cochromatography of radioactivity with standard bases. It is possible that this reaction may explain the presence of constitutive cellular enzymes specifically for the repair of methylated DNA, and may indicate the mechanism whereby methylated guanine is formed in the liver DNA of rats with chemically induced liver damage.