Comparison between Lumogallion and Calcon on Ion Pair Extraction-Fluorometry and the Application for Determination of Tertiary Amines

Abstract

Fluorescent intensity of lumogallion, a fluorescent complex formed by the addition of aluminum ion after association with primary, secondary and tertiary n-alkylamines was compared with that of calcon. Lumogallion formed a well-extractable complex with tertiary amines but merely produced a complex with secondary amines and did not produce a complex with primary amines. This method was applied to the determination of local anesthetics and psychotropic agents. Carpipramine, opipramol, benactyzine, chlorpromazine, procaine and lidocaine could be determined in the range of 2 .times. 10-8 to 10-9 mol/ml sample (.apprx. 8 .times. 10-10 to 10-11 mol/ml measurement solution). Primary and secondary amines at least less than equal molar in the sample did not interfere with the determination. Recoveries of carpipramine and chlorpromazine taken in milk were 99-103%.